Research of dissolved oxygen prediction in recirculating aquaculture systems based on deep belief network

Recirculating aquaculture has received more and more attention because of its high efficiency of treatment and recycling of aquaculture wastewater. The content of dissolved oxygen is an important indicator of control in recirculating aquaculture, its content and dynamic changes have great impact on the healthy growth of fish. However, changes of dissolved oxygen content are affected by many factors, and there is an obvious time lag between control regulation and effects of dissolved oxygen. To ensure the aquaculture production safety, it is necessary to predict the dissolved oxygen content in advance. The prediction model based on deep belief network has been proposed in this paper to realize the dissolved oxygen content prediction. A variational mode decomposition (VMD) data processing method has been adopted to evaluate the original data space, it takes the data which has been decomposed by the VMD as the input of deep belief network (DBN) to realize the prediction. The VMD method can effectively separate and denoise the raw data, highlight the relations among data features, and effectively improve the quality of the neural network input. The proposed model can quickly and accurately predict the dissolved oxygen content in time series, and the prediction performance meets the needs of actual production. When compared with bagging, AdaBoost, decision tree and convolutional neural network, the VMD-DBN model produces higher prediction accuracy and stability.     

循环水养殖中水动力特性对鱼类影响的研究进展

水产养殖业在我国拥有重要的地位。在过去50多年间,以中国为代表的水产养殖业的发展速度已超过全球人口增速,全球已经有39个国家和地区的水产养殖产量超过了捕捞产量,中国则以水产养殖产量占渔业总产量的73%位列第一[1-2]。随着经济的发展和养殖规模的不断提升,养殖业出现了生产方式落后、效率低下、养殖环境污染、水域生态破坏、病害频发等一系列亟待解决的问题[3-4]。     

温度对拟穴青蟹循环水养殖系统微生物群落结构的影响

为探究温度对拟穴青蟹(Scylla paramamosain)循环养殖水体中微生物群落的影响,深入理解环境因子与养殖水体微生物群落之间的相关性。在水温分别为18℃、22℃、26℃、30℃和32℃的循环养殖系统中进行拟穴青蟹养殖,采用16S rRNA高通量测序技术对不同温度下的养殖水体微生物群落组成进行分析。结果表明:(1) Alpha多样性指数显示5组水样中细菌群落多样性指数大小为t26t22t30t32t18,物种丰度大小为t22t26t32t30t18。温度可以导致养殖水体中微生物群落多样性发生变化,但并未发现二者之间直接的相关性。(2) 5种温度下的养殖系统中细菌群落共有31门、43纲、94目、185科和351属,变形菌门(Proteobacteria)为循环养殖水体中绝对优势菌门。在养殖系统水温为26℃时,养殖水体中氮磷污染物含量相对较高,其水中脱氮除磷菌属的相对丰度也显著高于其他4组。(3)由花瓣图和UPGMA聚类分析图进行相似性分析可知,水体微生物群落对温度的变化有着良好的响应, 5组水样的细菌群落结构总体相似性较低,其中最高温度32℃和最低温度18℃的水体中细菌群落结构最为相似。(4)水体中微生物群落结构和与环境因子的相关性分析显示,氮磷营养盐中的总磷(TP)和硝酸盐(NO3–-N)对水中微生物群落特征具有显著性影响,而温度是导致养殖水体中氮磷营养盐差异化的主要原因。上述研究结果初步解释了温度对拟穴青蟹循环养殖水微生物群落的作用机制,为下一步深入研究拟穴青蟹循环水养殖系统中环境变化与微生物的相互关系提供参考。     

斑点叉尾鮰养殖池塘底泥微生物群落结构特征及其影响因素

微生物群落在水产养殖环境中起着重要作用，深入了解微生物群落组成及其构建机制对了解池塘生态功能具有重要意义。本研究通过高通量测序技术研究斑点叉尾鮰（）养殖池塘底泥中细菌群落机构特征，并分析了细菌群落的主要影响因素。结果显示，斑点叉尾鮰养殖池塘底泥细菌群落结构呈现出季节性变化，冬春季样品相似性较高。斑点叉尾鮰养殖池塘底泥优势菌群主要为变形菌门（Proteobacteria）、绿弯菌门（Chloroflexi）、拟杆菌门（Bacteroidetes）、厚壁菌门（Firmicutes）和酸杆菌门（Acidobacteria），与池塘水体细菌群落间有明显的差别。线性判别分析显示，冬、春和秋季的特异性优势菌群较为丰富，冬季和春季分布较为集中，分别集中于浮霉菌、拟杆菌门、绿弯菌门和厚壁菌门、拟杆菌门，秋季特异性优势菌群分布特别分散，而夏季优势细菌类群最少，只有绿弯菌门厌氧绳菌属1个属具有显著优势。环境因子中，透明度和总溶解性悬浮物与斑点叉尾鮰养殖池塘底泥细菌群落结构有显著相关（<0.05），对细菌群落结构的影响也最大。本研究旨为斑点叉尾鮰池塘微生物群落的变化和调控提供借鉴。     

添加芽孢杆菌对草鱼池塘中真核微生物的影响

为了研究添加芽孢杆菌对池塘中真核微生物群落结构和理化因子的影响,采用高通量测序技术分析了实验组(添加芽孢杆菌池塘)与对照组(普通池塘)水体真核微生物群落结构,同时分析了两组池塘的水体理化指标。结果表明:8、9月实验组池塘水体中TN、NH_4~+-N、NO_3~--N含量显著低于对照组(P0.05)。水体中梅尼小环藻(Cyclotella meneghiniana)、红囊藻(Hedriocystis)、蓝隐藻(Chroomonas)、丝孢酵母属(Trichosporon)和小环藻属(Cyclotella)真核微生物丰度显著高于对照组(P0.05)。实验组池塘水体真核微生物Chao1指数和Shannon指数显著高于对照组(P0.05)。实验结果证实:通过向池塘添加芽孢杆菌,可以改变水体中真核微生物群落的结构,从而实现对池塘理化因子的调节。研究结果对于降低水产养殖尾水对水域环境的污染具有一定的意义。     

Hydrodynamic method for estimating production carrying capacity of coastal finfish cage aquaculture in Southeast Asia

This paper presents the development of a simple and generally applicable hydrodynamic method for the estimation of production carrying capacity (PCC) of coastal finfish cage aquaculture. Dimensional analysis was used to find significant and general interdependencies between the hydrodynamics at fish farm locations and particulate wastes deposited on the seafloor by fish farms. Modeled ratios of deposition to emission of particulate wastes underneath fish farms were found to be primarily a function of the flow Reynolds numbers at the farming locations and the non-dimensional settling velocity of emitted wastes. In the non-dimensional model, farming conditions include daily feed rate, proportion of unconsumed feed, and carbon content in feed and fish feces. The relationship can be used to estimate the PCC of floating net cages imposing a threshold value for deposition. Results of in-situ assessments of the benthic impacts of several fish farms in an aquaculture site in the northwest of Bali, Indonesia were used to validate and demonstrate the effectiveness of the method. Predicted results were able to clearly identify fish farms operating beyond ecologically sustainable carrying capacity. The proposed method has broad applicability and could help make decisions regarding the estimation of production potential of individual farms in pristine areas, for providing first estimates in sites that have scarce data, and for assessment, expansion, and optimization of the currently operating aquaculture sites in Southeast Asia, China and potentially other data-poor island nations. As the method relies on dynamic models, it enables straightforward assessments over the entire aquaculture region.     

南北接力养殖对皱纹盘鲍营养成分的影响

鲍的南北接力养殖是我国的一种常见养殖模式,旨在提高南方鲍鱼在夏季的存活率。鲍鱼通常在4月份从中国南方运至北方,11月返回南方。本实验探讨了这种模式对皱纹盘鲍营养成分的影响。测定方法主要依据GB 5009系列。研究样品于2017年12月采样,为相同饵料喂养(龙须菜)的商品鲍。两组鲍分别为全年于南方养殖(连江)的皱纹盘鲍和南北接力养殖的皱纹盘鲍。结果发现,两组鲍足肌中灰分、胶原蛋白、粗脂肪和糖原含量并没有显著差异,但南北接力组(水分:76.50%WW,蛋白质48.40%DW)相比于全年于南方养殖组(水分:73.70%WW,蛋白质:56.80%DW)有较高的水分含量和更低的蛋白质含量。矿物质含量方面,全年于南方养殖的皱纹盘鲍(0.07 mg/100g)足肌中硒的含量高于南北接力养殖组(0.05 mg/100 g)。呈味氨基酸方面,南北接力养殖方式下的皱纹盘鲍,其足肌谷氨酸、牛磺酸、精氨酸、赖氨酸和呈味氨基酸总量显著低于全年南方养殖组。脂肪酸方面,两组鲍有相似的脂肪酸组成,但南北接力养殖组的脂肪酸营养价值较高。研究表明,南北接力养殖模式对皱纹盘鲍的营养成分既有积极影响,又有消极影响,但总体上看差别并不显著。     

Pectic hydrolysates in the diet of silver catfish (Rhamdia quelen): Growth performance,blood and liver biochemistry,histological parameters and intestinal contents

The aim of this study was to investigate the prebiotic potential of pectin hydrolysates that were extracted from agroindustrial waste (apple pomace—AP and passion fruit peel—PFP) and were added to the diet of silver catfish (Rhamdia quelen). A 49‐day biological assay was conducted, and five test diets were evaluated: one diet was a control diet and the other four diets included pectin hydrolysates (2.5 and 5 g/kg). At 49 experimental days, biometric data and biological material were collected to determine the performance, plasma and liver and histological parameters and to evaluate the intestinal contents. The results were analysed by the normality test, which was followed by an analysis of the variance; the treatment means were compared by an orthogonal contrast analysis at a 5% level of significance. The inclusion of 2.5 g/kg apple pomace hydrolysates resulted in a greater production of butyric acid, increased thickness of the muscular layer and higher goblet cell count in the intestine. The inclusion of 5 g/kg apple pomace hydrolysates led to a greater concentration of liver protein. Further studies are needed to increase the knowledge about the use of these additives in the diet of silver catfish and to establish levels that allow greater gains for the species.     

Aurantiochytrium sp. meal as DHA source in Nile tilapia diet,part II: Body fatty acid retention and muscle fatty acid profile

Nile tilapia juveniles (8.35 ± 0.80 g) were fed on four levels (0.0%; 0.5%; 1.0%; 2.0%, 4.0%) of Aurantiochytrium sp. meal (ALL‐G‐RICH?), a source of docosahexaenoic acid (DHA). The 1% Aurantiochytrium sp. meal diet was compared to a control diet, which contained the same amount of DHA as cod liver oil (CLO) at 1.7% diet. Groups of 25 fish were stocked in 100 L tanks and fed twice daily until apparent satiation, for 57 days, at 28°C. Increasing dietary Aurantiochytrium sp. meal reduced the body retention of DHA and n‐3 polyunsaturated fatty acids (n‐3 PUFA) but increased the body retention of alpha‐linolenic (α‐LNA), linoleic (LOA) and n‐6 polyunsaturated fatty acids (n‐6 PUFA). Fatty acid profile in tilapia muscle was affected by increasing dietary inclusions of Aurantiochytrium sp. meal, with an increase in DHA, α‐LNA, n‐3 PUFA and n‐3 long chain‐polyunsaturated fatty acids (n‐3 LC‐PUFA) but a decrease in monounsaturated fatty acids (MUFA), n‐6 PUFA and n‐6 long‐chain polyunsaturated fatty acids (n‐6 LC‐PUFA). There was a larger body retention of DHA, α‐LNA, LOA, n‐3 PUFA and n‐6 PUFA fatty acids and a higher percentage of DHA, n‐3 PUFA and n‐3 LC‐PUFA in muscle fatty acid profile in fish fed on CLO diets than in those fed on 1% Aurantiochytrium sp. Therefore, Aurantiochytrium sp. meal is an alternative source of DHA for Nile tilapia diets.     

Observation of three‐dimensional flow structures and effluent transport around fish cages using a towed ADCP and free‐fall multi‐parameter profiler

A field campaign was conducted around salmon cages, using a combination of a towed ADCP and a free‐fall multi‐parameter profiler, in order to investigate flow structures and the possible distribution of effluent materials. Two transect observations showed that hydrographic conditions changed dramatically within 5 days, from highly stratified open water conditions to weak stratification. Three‐dimensional observation revealed that flow was blocked behind the cages and that the blockage was reduced as the distance from the cages increased. The flow speed was positively correlated with the intensity of the backscattering signal. The R² value was high immediately behind the cages and decreased with distance from the cages. The flow time series behind the cages exhibited a k^–2 power law spectrum that was consistent with a typical internal wave spectrum. This suggests that eddies shed from the cages were highly influenced by stratification. We estimated the rate of kinetic energy dissipation, , from YODA Profiler data based on the Thorpe scale approach. We also estimated the eddy diffusivity coefficient, . Both and followed a lognormal probability density function. The mean was consistent with a one‐dimensional diffusion model assessed from the R² values of flow speed and backscatter intensity.